![]() ![]() * These buffers can be used for all gel types and formulations.Ī variety of agarose powders are available. Northern and Southern blotting prehybridization and hybridization solutions ![]() Western blotting blocking buffer (casein blockers recommended for all applications, including those with biotin-avidin complexes) Polyacrylamide or agarose gels extends the buffer capacity for longer DNA sequencing runsĤ0 mM Tris, 20 mM acetic acid, 1 mM EDTA, pH 8.0Ī discontinuous buffer system that increases transfer efficiency in semi-dry applications Protease analysis activates enzymes after electrophoresisĨ9 mM Tris, 89 mM boric acid, 2 mM EDTA, pH 8.3ġ30 mM Tris, 45 mM boric acid, 2.5 mM EDTA Protease analysis renatures enzymes after electrophoresis ![]() Therefore, TAE is the preferred buffer if the DNA will be used for cloning or ligation.Įlectrophoresis Buffer Selection Guide Buffer Linear double stranded DNA is separated faster in TAE buffer however, this buffer has a lower buffering capacity than TBE because the borate in the TBE buffer acts as an enzyme inhibitor and could negatively impact downstream applications. Casting gates offer the convenience of casting directly in the electrophoresis cells and are available in one specific size for each cell (see Casting Guide below).Īn electrophoresis buffer conducts an electric current across the electrophoresis chamber the most commonly used buffers for DNA electrophoresis are TAE or TBE. Gel casters offer the flexibility of accommodating several different-sized UV transparent (UVTP) gel trays and are available for the entire Sub-Cell product line. Two accessories are available for handcasting gels within the Sub-Cell family of electrophoresis cells. Handcasting offers options for a variety of gel sizes. * A 3% ReadyAgarose gel is easier to handle and gives the same performance as a standard 4% gel. Optimal Resolution Range of ReadyAgarose Gels Gel Percentage † Sample throughput value assumes 1–4 combs per gel. *** Sample throughput value assumes 1–2 combs per gel. This cell does not include casting gates, tray, or combs. ** Wide mini ReadySub-Cell GT is a wide Mini-Sub cell GT dedicated to running ReadyAgarose precast gels, gel size 15 x 10 cm sample throughput is 20, 32, 2 x 32, or 4 x 26. * Mini ReadySub-Cell GT is a Mini-Sub cell GT dedicated to running ReadyAgarose precast gels, gel size 7 x 10 cm sample throughput is 8, 12, or 2 x 8. Bio-Rad offers numerous precast gels and ReadyAgarose gels for your convenience.īio-Rad's Sub-Cell System Selection Guide A large selection of tools, including combs with different numbers of wells and different well thicknesses, or different sized UV trays and gel casters, are available for simplifying gel casting.įor convenience, many users purchase precast gels, which are available in different sizes and gel percentages and are prepared either with Tris-acetate- EDTA (TAE) or Tris-borate EDTA (TBE) buffer. Many users cast their own gels in fact, gels can be cast either directly in the gel box, using a gel caster, or in a UV tray on which the openings are closed off by adhesive tape. Bio-Rad offers multiple choices of gel boxes called Sub-cell systems to fit different applications. Mini formats are usually used for quick separations and a small number of samples large formats are ideal for restriction fragment length polymorphism (RFLP), Southern blotting, and PCR sample screening. Gel boxes are available in different sizes to accommodate different needs. ![]()
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